The peroxidase-catalyzed oxidation of NADH as an indicator reaction for repetitive determinations by sample injection in closed flow-through systems: The determination of LDH in blood serum
✍ Scribed by D.P. Nikolelis; Chih-duen C. Painton; Horacio A. Mottola
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 554 KB
- Volume
- 97
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
The air oxidation of reduced nicotinamide adenine dinucleotide (NADH), catalyzed by peroxidase, provides a useful "indicator reaction" for the determination of both NADH and serum lactate dehydrogenase (LDH). Application of this indicator reaction for repetitive determinations by sample injection into a continuously circulated reagent mixture, and by monitoring of oxygen depletion with an amperometric sensor, is described. At determination rates of 260 determinations/h, NADH and LDH have been determined in the range of 3.8 x 10m4 to 7.6 x 1O-3 M and 70 to 700 U/ml, respectively, and with relative errors and standard deviations (population) of about 2%. LDH has been determined in human blood serum and the injection procedure compared with a modified Wroblewski-Ladue procedure; the Pearson correlation coefficient was found to be 0.99, (40 samples). Recovery studies are also reported for LDH in serum calibration references and controls.