𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Development of an immunochromatographic assay for rapid detection of 1-Aminohydantoin in urine specimens

✍ Scribed by Yi Ping Xu; Li Qiang Liu; Qiu Shen Li; Chi Fang Peng; Wei Chen; Chuan Lai Xu

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
194 KB
Volume
23
Category
Article
ISSN
0269-3879

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

A rapid immunochromatographic assay was developed and validated for detection of 1‐aminohydantoin (AHD) in urine specimens. Colloidal gold‐labeled polyclonal antibody specific to AHD derivative was used as the marker; based on the competitive reactivity theory, the metabolite of nitrofurantoin after derivatization with benzaldehyde would compete with carboxyphenyl AHD derivative‐conjugated ovalbumin. The test strip could efficaciously detect the novel analyte with a visual detection limit of 10 ng mL^−1^ and high specificity. The reliability of the assay was determined by testing 80 standard samples comparing with enzyme‐linked immunosorbent assay. The semi‐quantitative detection was accomplished in less than 15 min with low cost, especially for requirements of rapid and simple screening. This is the first publication of an immunochromatographic assay for detection of nitrofuran residues. Copyright © 2008 John Wiley & Sons, Ltd.

📜 SIMILAR VOLUMES

Development of an immunochromatographic
Development of an immunochromatographic strip for the rapid detection of Toxoplasma gondii circulating antigens
✍ Yan-Hua Wang; Xue-Rui Li; Guang-Xiang Wang; Hong Yin; Xue-Peng Cai; Bao-Quan Fu; 📂 Article 📅 2011 🏛 Elsevier Science 🌐 English ⚖ 187 KB

We developed a rapid immunochromatographic strip (ICS) procedure that can detect circulating antigens in the blood of animals during the acute stage of toxoplasmosis. The aim of this study was to evaluate this test using sera from field samples and from experimentally infected animals. The sensitivi

Development of an immunomagnetic assay s
Development of an immunomagnetic assay system for rapid detection of bacteria and leukocytes in body fluids
✍ John G. Bruno; Hao Yu; John P. Kilian; Arnott A. Moore 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 622 KB

Immunomagnetic (IM) separation and concentration of specific target ligands or particles, such as bacteria or leukocytes, from complex mixtures, such as bone marrow, blood and other body fluids, is now a widely accepted technique. IM methodologies require high a n i t y antibodies or other receptors

Evaluation of an immunochromatography me
Evaluation of an immunochromatography method for rapid detection of noroviruses in clinical specimens in Thailand
✍ Aksara Thongprachum; Pattara Khamrin; Natthawan Chaimongkol; Rungnapa Malasao; S 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 60 KB

## Abstract Norovirus (NoV) is a causative agent of gastroenteritis in children and adults worldwide. Although reverse transcription‐polymerase chain reaction (RT‐PCR) has been accepted as the standard method for diagnosis of NoV infection, it requires well‐trained personnel and sophisticated equip

Development and comparison of molecular
Development and comparison of molecular assays for the rapid detection of the pandemic influenza A (H1N1) 2009 virus
✍ Wai-Yip Lam; Ting-Fan Leung; Nelson Lee; Jo L.K. Cheung; Apple C.M. Yeung; Yolan 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 101 KB 👁 1 views

## Abstract Human infection with the novel pandemic influenza A (H1N1) virus was first identified in April 2009. Two months later, the World Health Organization (WHO) had raised the pandemic level to phase 6. Rapid case identification is essential for prompt patient management and public health act

Development and evaluation of a rapid im
Development and evaluation of a rapid immunochromatographic test for mumps-specific IgM in oral fluid specimens and use as a matrix for preserving viral nucleic acid for RT-PCR
✍ Lenesha Warrener; Rimas Slibinskas; David Brown; Kestutis Sasnauskas; Dhanraj Sa 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 160 KB

## Abstract The use of oral fluid specimens for diagnosis of mumps by detection of virus‐specific IgM or nucleic acid is now well established. The utility of oral fluids would be improved further if testing could be performed closer to the patient. A near patient test (NPT) for the detection of mum

Development of an enzyme-linked immunoso
Development of an enzyme-linked immunosorbent assay for 2,3-dinor-6-keto-prostagland in F1α in urine using a monoclonal antibody
✍ Carmen Lindsay; Jean-Marie Moutquin; René C.-Gaudreault; Jean-Claude Forest 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 587 KB

## Objectives: To develop and validate an enzyme-linked immunosorbent assay (elisa) for measurement of urinary 2,3-dinor-6-keto-prostaglandin f1 alpha (2,3d6kpgf1 alpha) using a monoclonal antibody and a horseradish peroxidase-linked antigen. ## Design and methods: Assay validation included optim