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Development of an ELISA for vitellogenin in whole body homogenate of zebrafish (Danio rerio)

✍ Scribed by Henrik Holbech; Lene Andersen; Gitte I Petersen; Bodil Korsgaard; Knud L Pedersen; Poul Bjerregaard

Book ID
117794227
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
255 KB
Volume
130
Category
Article
ISSN
1532-0456

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Development and validation of an enzyme-
Development and validation of an enzyme-linked immunosorbent assay to measure vitellogenin in the zebrafish (Danio rerio)
✍ François Brion; Bente M. Nilsen; Janne K. Eidem; Anders Goksøyr; Jean Marc Porch 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 910 KB

## Abstract In this study, an enzyme‐linked immunosorbent assay (ELISA) was developed to quantify vitellogenin (Vtg) in zebrafish (__Danio rerio__). Zebrafish Vtg (zf‐Vtg) was purified from whole‐body homogenates of estradiol‐exposed zebrafish, and polyclonal antibodies against zf‐Vtg were raised.