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An enzyme-linked immunosorbent assay for rare minnow (Gobiocypris rarus) vitellogenin and comparison of vitellogenin responses in rare minnow and zebrafish (Danio rerio)

✍ Scribed by Tao Liao; Shiwei Jin; Fang-Xing Yang; Yang Hui; Ying Xu

Book ID
113891445
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
231 KB
Volume
364
Category
Article
ISSN
0048-9697

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✍ François Brion; Bente M. Nilsen; Janne K. Eidem; Anders Goksøyr; Jean Marc Porch 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 910 KB

## Abstract In this study, an enzyme‐linked immunosorbent assay (ELISA) was developed to quantify vitellogenin (Vtg) in zebrafish (__Danio rerio__). Zebrafish Vtg (zf‐Vtg) was purified from whole‐body homogenates of estradiol‐exposed zebrafish, and polyclonal antibodies against zf‐Vtg were raised.