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Development of an antibody-lectin enzyme immunoassay for fucosylated α-fetoprotein

✍ Scribed by Hiroaki Korekane; Tomoko Hasegawa; Akio Matsumoto; Noriaki Kinoshita; Eiji Miyoshi; Naoyuki Taniguchi

Book ID
116272363
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
666 KB
Volume
1820
Category
Article
ISSN
0304-4165

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A new mimetic enzyme immunoassay (MEIA) method for cY-1-fetoprotein (AFP) was developed using a mimetic enzyme Mn( III)-tetra( suIfophenyl)porphine (Mn-TPPS,) as a labelling reagent to catalyze the fluorescence reaction of 4-hydroxyphenylacetic acid (HPA) and hydrogen peroxide. In the sandwich assay