Development of a β-galactosidase α-complementation system for molecular cloning in Bacillus subtilis
✍ Scribed by Haima, Peter; van Sinderes, Douwe; Schotting, Hans; Bron, Sierd; Venema, Gerard
- Book ID
- 121294319
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 708 KB
- Volume
- 86
- Category
- Article
- ISSN
- 0378-1119
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
A versatile plasmid marker rescue transformation system was developed for homology-facilitated cloning in Bacillus subtilis. It is based on the highly efficient host-vector system 6GM15-pHPS9, which allows the direct selection of recombinants by means of beta-galactosidase alpha-complementation. The
## Abstract This article describes a simple and robust laboratory exercise on the regulation of membrane unsaturated fatty acid composition in bacteria by a decrease in growth temperature. We take advantage of the well characterized Des pathway of __Bacillus subtilis__, composed of a Δ5‐desaturase