𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Development of a β-galactosidase α-complementation system for molecular cloning in Bacillus subtilis

✍ Scribed by Haima, Peter; van Sinderes, Douwe; Schotting, Hans; Bron, Sierd; Venema, Gerard


Book ID
121294319
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
708 KB
Volume
86
Category
Article
ISSN
0378-1119

No coin nor oath required. For personal study only.


📜 SIMILAR VOLUMES


Novel plasmid marker rescue transformati
✍ Haima, Peter ;Bron, Sierd ;Venema, Gerard 📂 Article 📅 1990 🏛 Springer 🌐 English ⚖ 808 KB

A versatile plasmid marker rescue transformation system was developed for homology-facilitated cloning in Bacillus subtilis. It is based on the highly efficient host-vector system 6GM15-pHPS9, which allows the direct selection of recombinants by means of beta-galactosidase alpha-complementation. The

Thermal regulation of membrane lipid flu
✍ L.M. Bredeston; D. Marciano; D. Albanesi; D. De Mendoza; J. M. Delfino 📂 Article 📅 2011 🏛 The American Society for Biochemistry and Molecula 🌐 English ⚖ 436 KB 👁 1 views

## Abstract This article describes a simple and robust laboratory exercise on the regulation of membrane unsaturated fatty acid composition in bacteria by a decrease in growth temperature. We take advantage of the well characterized Des pathway of __Bacillus subtilis__, composed of a Δ5‐desaturase