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An improved β-galactosidase α-complementation system for molecular cloning in Bacillus subtilis

✍ Scribed by Haima, Peter; van Sinderen, Douwe; Bron, Sierd; Venema, Gerard


Book ID
121891977
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
739 KB
Volume
93
Category
Article
ISSN
0378-1119

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Novel plasmid marker rescue transformati
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A versatile plasmid marker rescue transformation system was developed for homology-facilitated cloning in Bacillus subtilis. It is based on the highly efficient host-vector system 6GM15-pHPS9, which allows the direct selection of recombinants by means of beta-galactosidase alpha-complementation. The