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Development of a solid phase extraction method for simultaneous determination of corticoids and tranquilizers in serum samples

✍ Scribed by M. Carmen Quintana; Lourdes Ramos; M. Jose González; Manuel H. Blanco; Lucas Hernández

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
562 KB
Volume
27
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

A simple and fast solid phase extraction (SPE) method allowing the preconcentration, clean‐up, and subsequent separate elution of phenothiazines (chlorpromazine hydrochloride, acetopromazine, and propionylpromazine hydrochloride) and glucocorticoids (dexamethasone, betamethasone acetate, and phenylbutazone) from serum samples has been developed. Both fractions were separately collected and analyzed without any additional treatment by high performance liquid chromatography with UV‐Vis. The performance of the complete procedure was satisfactory irrespective of the spiking level with recoveries in the range 64–85% for all analytes investigated but for phenylbutazone (20%). Repeatability, evaluated as the relative standard deviation, was globally better than 12%. LC‐MS was used for final confirmation of the results.

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