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Development of a fluorescence polarization binding assay for asialoglycoprotein receptor

✍ Scribed by Anna Y. Kornilova; Bethany Algayer; Michael Breslin; Victor Uebele

Book ID
113412431
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
415 KB
Volume
425
Category
Article
ISSN
0003-2697

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Peptidyl-tRNA hydrolase (Pth) activity ensures the rapid recycling of peptidyl-tRNAs that result from premature termination of translation. Historically, the hydrolyzing activity of Pth has been assayed with radiolabeled N-blocked aminoacyl-tRNAs in assay systems that require the separation of radio