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A fluorescence polarization binding assay to identify inhibitors of flavin-dependent monooxygenases

✍ Scribed by Jun Qi; Karina Kizjakina; Reeder Robinson; Karishma Tolani; Pablo Sobrado


Book ID
113412447
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
640 KB
Volume
425
Category
Article
ISSN
0003-2697

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Ribonuclease A (RNase A) and the ribonuclease inhibitor protein (RI) form one of the tightest known protein-protein complexes. RNase A variants and homologues, such as G88R RNase A, that retain ribonucleolytic activity in the presence of RI are toxic to cancer cells. Herein, a new and facile assay i