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Development of a duplex real-time PCR for the detection of Rickettsia spp. and typhus group rickettsia in clinical samples

✍ Scribed by Stefano Giulieri; Katia Jaton; Alain Cometta; Laurence T. Trellu; Gilbert Greub


Book ID
109309851
Publisher
John Wiley and Sons
Year
2012
Tongue
English
Weight
103 KB
Volume
64
Category
Article
ISSN
0928-8244

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## Abstract A two‐step reverse transcriptase TaqManβ„’ duplex PCR (RT‐PCR) assay was developed using the ABI 7700 Sequence Detection System for the detection of enterovirus (EV) and parechovirus type 1 and 2 (PEV) RNA from samples of cerebrospinal fluid (CSF) and throat swabs. Using sequence‐specific