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Development and validation of a real-time TaqMan PCR assay for the detection of betanodavirus in clinical specimens

โœ Scribed by V. Panzarin; P. Patarnello; A. Mori; E. Rampazzo; E. Cappellozza; G. Bovo; G. Cattoli


Publisher
Springer Vienna
Year
2010
Tongue
English
Weight
571 KB
Volume
155
Category
Article
ISSN
1432-8798

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Since the characterization of the genome of the hepatitis E virus (HEV) in 1990, a large genetic diversity has been described. A single real-time reverse transcription (RT)-PCR assay with TaqMan technology has been validated which uses only one set of primers and probe within the ORF2 HEV region (nt