Development and validation of an RP-HPLC-UV method for the determination of BOL-303225-A, a new coumarin-based anti-inflammatory drug, in rat plasma

✍ Scribed by Adriana Maltese; Francesco Maugeri; Keith W. Ward; Claudio Bucolo

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 181 KB
• Volume: 21
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/bmc.757

✦ Synopsis

Abstract

A simple and rapid high‐performance liquid chromatographic method was developed and validated for the analysis in rat plasma of BOL‐303225‐A, a new coumarin‐based anti‐inflammatory drug. Liquid–liquid extraction was used for sample preparation. Chromatographic separation was achieved on a C~18~ column using acetonitrile and water containing 1% triethylamine pH 3.5, adjusted with orthophosphoric acid (35.5:64.5 v/v) as mobile phase. The UV detector was set at 324 nm. The method proved to be linear (r^2^ > 0.99) and precise (RSD < 7%) over the concentration range 29–940 ng/mL, and was suitable for the support of pharmacokinetic studies in rats. Copyright © 2007 John Wiley & Sons, Ltd.