๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Development and validation of an RP-HPLC method for quantitative determination of vanillin and related phenolic compounds in Vanilla planifolia

โœ Scribed by Arun Kumar Sinha; Subash Chandra Verma; Upendra Kumar Sharma

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
702 KB
Volume
30
Category
Article
ISSN
1615-9306

No coin nor oath required. For personal study only.

โœฆ Synopsis

Development and validation of an RP-HPLC method for quantitative determination of vanillin and related phenolic compounds in Vanilla planifolia

A simple and fast method was developed using RP-HPLC for separation and quantitative determination of vanillin and related phenolic compounds in ethanolic extract of pods of Vanilla planifolia. Ten phenolic compounds, namely 4-hydroxybenzyl alcohol, vanillyl alcohol, 3,4-dihydroxybenzaldehyde, 4-hydroxybenzoic acid, vanillic acid, 4-hydroxybenzaldehyde, vanillin, p-coumaric acid, ferulic acid, and piperonal were quantitatively determined using ACN, methanol, and 0.2% acetic acid in water as a mobile phase with a gradient elution mode. The method showed good linearity, high precision, and good recovery of compounds of interest. The present method would be useful for analytical research and for routine analysis of vanilla extracts for their quality control.

๐Ÿ“œ SIMILAR VOLUMES

RP-HPTLC densitometric determination and
RP-HPTLC densitometric determination and validation of vanillin and related phenolic compounds in accelerated solvent extract of Vanilla planifolia*
โœ Upendra Kumar Sharma; Nandini Sharma; Ajai Prakash Gupta; Vinod Kumar; Arun Kuma ๐Ÿ“‚ Article ๐Ÿ“… 2007 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 659 KB

## Abstract A simple, fast and sensitive RPโ€HPTLC method is developed for simultaneous quantitative determination of vanillin and related phenolic compounds in ethanolic extracts of __Vanilla planifolia__ pods. In addition to this, the applicability of accelerated solvent extraction (ASE) as an alt

Development and validation of an RP-HPLC
Development and validation of an RP-HPLC method for the estimation of adenosine and related purines in brain tissues of rats
โœ Kiran Kumar Akula; Manninder Kaur; Mahendra Bishnoi; Shrinivas K. Kulkarni ๐Ÿ“‚ Article ๐Ÿ“… 2008 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 470 KB

## Abstract A new, rapid and sensitive RPโ€HPLC method with UV spectrophotometric detection was developed and validated for the concomitant estimation of adenosine and related purines in rat brain tissue preparations. The HPLC system consisted of Cโ€18 column with UVโ€“photodiodeโ€array detection rangin

Development and validation of an RP-HPLC
Development and validation of an RP-HPLC-UV method for the determination of BOL-303225-A, a new coumarin-based anti-inflammatory drug, in rat plasma
โœ Adriana Maltese; Francesco Maugeri; Keith W. Ward; Claudio Bucolo ๐Ÿ“‚ Article ๐Ÿ“… 2007 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 181 KB ๐Ÿ‘ 3 views

## Abstract A simple and rapid highโ€performance liquid chromatographic method was developed and validated for the analysis in rat plasma of BOLโ€303225โ€A, a new coumarinโ€based antiโ€inflammatory drug. Liquidโ€“liquid extraction was used for sample preparation. Chromatographic separation was achieved on

Development and validation of an HPLC me
Development and validation of an HPLC method for the determination of seven penicillin antibiotics in veterinary drugs and bovine blood plasma
โœ Victoria F. Samanidou; Dimitrios E. Giannakis; Antonia Papadaki ๐Ÿ“‚ Article ๐Ÿ“… 2009 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 658 KB

## Abstract Herein a quantitative method for the determination of seven penicillins in bovine plasma and veterinary drugs has been developed. Amoxicillin (AMO), ampicillin (AMP), penicillin G (PENG), penicillin V (PENV), oxacillin (OXA), cloxacillin (CLO) and dicloxacillin (DICLO) were separated on