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Development and validation of a triplex real-time PCR assay for the rapid detection and differentiation of wild-type and glycoprotein E-deleted vaccine strains of Bovine herpesvirus type 1

✍ Scribed by Kerstin Wernike; Bernd Hoffmann; Donata Kalthoff; Patricia König; Martin Beer

Book ID: 113768662
Publisher: Elsevier Science
Year: 2011
Tongue: English
Weight: 574 KB
Volume: 174
Category: Article
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2011.03.028

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## Abstract A real‐time PCR assay was developed to identify varicella‐zoster virus (VZV) and herpes simplex virus (HSV) DNA in clinical specimens from subjects with suspected herpes zoster (HZ; shingles). Three sets of primers and probes were used in separate PCR reactions to detect and discriminat