## Abstract A realβtime PCR assay was developed to identify varicellaβzoster virus (VZV) and herpes simplex virus (HSV) DNA in clinical specimens from subjects with suspected herpes zoster (HZ; shingles). Three sets of primers and probes were used in separate PCR reactions to detect and discriminat
A real-time PCR assay for the detection of varicella-zoster virus DNA and differentiation of vaccine, wild-type and control strains
β Scribed by Graham A. Tipples; David Safronetz; Michael Gray
- Book ID
- 114140143
- Publisher
- Elsevier Science
- Year
- 2003
- Tongue
- English
- Weight
- 73 KB
- Volume
- 113
- Category
- Article
- ISSN
- 0166-0934
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In Japan and the United States, where vaccination against varicella-zoster virus (VZV) infection with the live attenuated Oka strain of varicella is routine, cases of chickenpox or shingles occurring in vaccinees can be caused by either wildtype or vaccine virus. Differentiating such cases is import
## Abstract One hundred fortyβnine specimens were tested in a LightCycler nested multiplex polymerase chain reaction (LCnmPCR) for Herpes simplex virus (HSV)1, HSV2, and VZV. Eightyβone were from genitourinary medicine (GUM) patients and the other 68 specimens were from other patients with skin les