Development and Validation of a High-performance Liquid Chromatography Method for the Determination of Cocaine, its Metabolites and Lidocaine

✍ Scribed by Stephen A. Barat; Stacey A. Kardos; Mohamed S. Abdel-Rahman

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 387 KB
• Volume: 16
• Category: Article
• ISSN: 0260-437X
• DOI: 10.1002/(sici)1099-1263(199605)16:3<215::aid-jat335>3.0.co;2-i

✦ Synopsis

Street cocaine varies in purity and is often adulterated with various compounds. Some of these additives, such as lidocaine, may increase the toxicity of cocaine. A new precise, accurate and sensitive reversedphase high-performance liquid chromatography method for the determination of cocaine, its metabolites and lidocaine in plasma samples has been developed and validated. This assay employed a phosphatebuffered aqueous mobile phase (pH 6.0) with an organic component consisting of acetonitrile and methanol and a C-18 column as the stationary phase. Minimum detection limits were 1 ng ml-' for cocaine, 2.5 ng ml-I for ethylcocaine and 5 ng ml-' for benzoylecgonine, norcocaine, norethylcocaine and lidocaine. Linearity was demonstrated over a broad range of concentrations in plasma, with good sensitivity for cocaine and cocaine derivatives.