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Development and mapping of microsatellites from a microdissected BTA 11-specific DNA library

✍ Scribed by S. Ambady; S. M. Kappes; C. Park; R. Z. Ma; J. E. Beever; H. A. Lewin; T. P. L. Smith; C. W. Beattie; P. K. Basrur; F. Abel Ponce De León

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
89 KB
Volume
32
Category
Article
ISSN
0268-9146

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✦ Synopsis

A chromosome‐specific library was developed for Bos taurus autosome 11 by chromosome microdissection and microcloning using a bovine primary fibroblast culture, obtained from a t(X;23) heifer, that spontaneously developed a translocation chromosome involving bovine chromosome 11. The library was screened using (AC)~12~ oligos, positive clones selected, sequenced and primers developed to generate bovine chromosome 11‐specific microsatellite markers. This study suggests that chromosome‐specific libraries have great potential for development of microsatellite markers for the construction of marker‐saturated linkage maps for each chromosome.

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