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Mapping of rabbit chromosome 1 markers generated from a microsatellite-enriched chromosome-specific library

✍ Scribed by R. Korstanje; G. F. Gillissen; M. G. Den Bieman; S. A. Versteeg; B. Van Oost; R. R. Fox; H. A. Van Lith; L. F. M. Van Zutphen

Publisher: John Wiley and Sons
Year: 2001
Tongue: English
Weight: 108 KB
Volume: 32
Category: Article
ISSN: 0268-9146
DOI: 10.1046/j.1365-2052.2001.00783.x

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✦ Synopsis

A genomic DNA library was produced from flow‐sorted rabbit chromosome 1 and enriched for fragments containing CA‐repeats. Clones containing CA‐repeats were identified and primers for amplification of the microsatellite were developed after sequencing the clone. The degree of polymorphism was tested in rabbits from different breeds. This approach identified 12 microsatellite markers which could be used for studying linkage relationships in the progeny of an F~2~‐intercross: (AX/JUxIIIVO/JU) F~2~, and two backcrosses: (OS/JxX/J)X/J and (WH/JxX/J)X/J. Seven of these markers were mapped on chromosome 1.

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