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Development and life cycle of the parthenogenetically activated sea urchin embryo

✍ Scribed by Brandriff, B. ;Hinegardner, R. T. ;Steinhardt, R.

Publisher
John Wiley and Sons
Year
1975
Tongue
English
Weight
872 KB
Volume
192
Category
Article
ISSN
0022-104X

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✦ Synopsis

Abstract

A method is reported for inducing parthenogenetic development in eggs of the sea urchin Lytechinus pictus, a species which previously could not be artificially activated. NH~4~OH or the calcium ionophore A23187 are used as activating agents followed by hypertonic treatment. The ionophore is superior in activating large numbers of unfertilized eggs, whereas NH~4~OH produces a larger percent of embryos able to undergo gastrulation. Both feeding larvae and urchins arising from these artificially activated eggs are diploid. All individuals in which sex has been identified have been female. The viability of these completely homozygous organisms is low compared to their fertilized counterparts.

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