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Inhibition by aphidicolin of cell cycle progression and DNA replication in sea urchin embryos

✍ Scribed by Susumu Ikegami; Shonan Amemiya; Mieko Oguro; Hiroshi Nagano; Yoshitake Mano

Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
428 KB
Volume
100
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

We have recently found that aphidicolin, a tetracyclic diterpene‐tetraol produced by several fungi, blocks DNA synthesis of sea urchin embryos by interfering with the activity of DNA polymerase α. These cells fail to proliferate in the presence of aphidicolin.

In continuation of these studies, we determined the drug‐sensitive stage in the first cell cycle of the sea urchin Clypeaster japonicus embryo.

In continuous exposure to aphidicolin (2 μg/ml) from five minutes after fertilization, mitotic division of the embryo was completely suppressed. Embryos were exposed to the drug at progressively later intervals and their capability for cytokinesis was examined. Evidence was thereby obtained that aphidicolin acts at the S‐period to inhibit DNA synthesis resulting in developmental arrest of the embryo.

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