Development and evaluation of method for simultaneous determination of phenobarbital and diphenylhydantoin in plasma by high-pressure liquid chromatography

✍ Scribed by S. H. Atwell; V. A. Green; W. G. Haney

• Publisher: John Wiley and Sons
• Year: 1975
• Tongue: English
• Weight: 420 KB
• Volume: 64
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600640516

✦ Synopsis

Abstract0 A procedure for the simultaneous analysis of diphenylhydantoin and phenobarbital in plasma by high-pressure liquid chromatography was developed and evaluated. Separation is effected on a porous particle silicic acid column with chloroformdioxane-isopropanol-acetic acid (3109.7:1.00.1 by volume) and is monitored at 254 nm. Results of the procedure were compared with results of a GLC assay.

Keyphrases

Phenobarbital-diphenylhydantoin-simultaneous high-pressure liquid chromatographic determination, plasma Diphenylhydantoin-phenobarbital-simultaneous high-pressure 'liquid chromatographic determination, plasma 0 High-pressure liquid chromatography-simultaneous determination of phenobarbital and diphenylhydantoin in plasma, development and evaluation of method ALC 202, Waters Associates, Milford, Mass.