Development and evaluation of data-driven designed tags (DDTs) for controlling protein solubility

✍ Scribed by Shuichi Hirose; Yoshifumi Kawamura; Masatoshi Mori; Kiyonobu Yokota; Tamotsu Noguchi; Naoki Goshima

• Book ID: 103851129
• Publisher: Elsevier
• Year: 2011
• Tongue: English
• Weight: 625 KB
• Volume: 28
• Category: Article
• ISSN: 1871-6784
• DOI: 10.1016/j.nbt.2010.08.012

✦ Synopsis

Production of proteins is an important issue in protein science and pharmaceutical studies. Numerous protein expression systems using living cells and cell-free methods have been developed to date. In these systems, a promising strategy for improving the success rate of obtaining soluble proteins is the attachment of various tags into target proteins based on empirical rules. This paper presents a method for the production of data-driven designed tags (DDTs) based on highly frequent sequence property patterns in an experimentally assessed protein solubility dataset in a wheat germ cell-free system. We constructed seven proteins combined with 12 kinds of DDTs (six for enhancing solubility and six for insolubility) at the N-terminal region as tags. Then we investigated their behavior using SDS-PAGE. Results show that three and four proteins respectively showed a trend toward solubilization and insolubilization, which indicates the possibility that the theoretically designed sequence can control protein solubility.