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Development and evaluation of a CERES-type model for winter oilseed rape

✍ Scribed by B. Gabrielle; P. Denoroy; G. Gosse; E. Justes; M.N. Andersen

Book ID
117530093
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
485 KB
Volume
57
Category
Article
ISSN
0378-4290

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A method is described for regenerating callus from mesophyll protoplasts of a winter variety of Brassica napus. The method combines the use of Ficoll in an initial liquid medium, enhancing early protoplast division and cell colony formation, with a transfer to an agarose system after 10 days culture