✦ LIBER ✦
Effects of a Ficoll-agarose system on early division and development of mesophyll protoplasts of winter oilseed rape (Brassica napusL.)
✍ Scribed by Stephen R. Millam; Alan T. H. Burns; Trevor J. Hocking; Ken J. Cattell
- Publisher
- Springer
- Year
- 1988
- Tongue
- English
- Weight
- 262 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0167-6857
No coin nor oath required. For personal study only.
✦ Synopsis
A method is described for regenerating callus from mesophyll protoplasts of a winter variety of Brassica napus. The method combines the use of Ficoll in an initial liquid medium, enhancing early protoplast division and cell colony formation, with a transfer to an agarose system after 10 days culture to give rapid microcaUi formation. Further transfers resulted in callus regeneration and the initiation of organogenesis.