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Determining protein kinase substrate specificity by parallel solution-phase assay of large numbers of peptide substrates

✍ Scribed by Turk, Benjamin E.; Hutti, Jessica E.; Cantley, Lewis C.


Book ID
109946067
Publisher
Nature Publishing Group
Year
2006
Tongue
English
Weight
245 KB
Volume
1
Category
Article
ISSN
1750-2799

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Method for Determining Protein Kinase Su
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A method is described for the elucidation of the peptide substrate phosphorylation specificity of a protein kinase. Peptide libraries with two to six degenerate positions and a length of seven or nine amino acids were generated directly on Sepharose beads by solidphase peptide synthesis according to