Determination of β-phenylethylamine concentrations in human plasma, platelets, and urine and in animal tissues by high-performance liquid chromatography with fluorometric detection

✍ Scribed by Motohiro Tsuji; Ken Ohi; Chiaki Taga; Tetsuro Myojin; Saburo Takahashi

• Publisher: Elsevier Science
• Year: 1986
• Tongue: English
• Weight: 449 KB
• Volume: 153
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(86)90068-0

✦ Synopsis

A highly sensitive method for the determination of &phenylethylamine in human plasma, platelets, and urine and in mouse tissue is described. The method is based on a two-step isolation using cation-exchange columns followed by reversephase high-performance liquid chromatography with fluorometric detection. The recovery of the amine through the whole procedure was almost complete, ranging from 99 to 101%. The calibration graph appeared linear over the range of 50 to 5000 pg/injection. Urinary excretion of fl-phenylethylamine in humans ranged from 0.93 to 5 1.20 ng/mg creatinine. The amine was also detectable in plasma and platelets. Of the various mouse tissues examined, the highest concentrations were found in the small intestine, followed by the blood and liver. Concentrations of about 5 ndg wet wt were detected in brain tissue, which increased remarkably after inhibition of monoamine oxidase by pargyline. Q 1986 Academic