Determination of urinary oxalate and porphyrins by peroxyoxalate chemiluminescence

✍ Scribed by Steffen Albrecht; Herbert Brandl; Wolf-Dieter Böhm; Rainer Beckert; Herbert Kroschwitz; Volker Neumeister

• Publisher: Elsevier Science
• Year: 1991
• Tongue: English
• Weight: 281 KB
• Volume: 255
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(91)80076-6

✦ Synopsis

The apphcatlon of the chemdummescence system oxahc acid-fluorescer-peroxy compound-carbodnnude to bmchenucal analyst m prow solvents IS described By measunng the mtegrated chennlummescence enussion generated by the above reactlon, very low concentrations of oxalate (down to 10 nmol I-') can be determmed Optmuzed reamon con&tlons and preanalytlcal factors pernut direct and rapld measurements of unnary oxalate wthout calcmm oxalate preclpltatlon The system also pernuts rapld quantltatwe analysis for urmary porphyrms down to about 200 p.g 1-l Keywords Cheuulunuuescence, O&ate, Porphyrins, Unne Chemdummescent systems based on lummol, on the apphcatlon of the currently most efflclent acndme, phenanthndme and lophme dematmes chemllummescent system, peroxyoxalate chemduand smglet oxygen-generatmg hypohahte-peroxy mmescence (CL), wth CL quantum yields up to compound systems have been widely used m ana-0 34 Emstem mol-' m aprotlc solvents Oxalic &xl bmchemlstry [ll There are fewer reports acid esters or oxarmdes, which are poorly soluble