Determination of urinary free cortisol and cortisone by sequential thin layer and high-performance liquid chromatography
β Scribed by Marvin L. Lewbart; Robert A. Elverson
- Book ID
- 116002592
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 626 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0022-4731
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π SIMILAR VOLUMES
A new method for the simultaneous determination of cortisol and cortisone in serum by high-performance liquid chromatography with fluorimetric detection has been developed. The two steroids were derivatized by treatment with 9-anthroyl nitrile in triethylamine-acetonitrile to give the fluorescent es
## Abstract A rapid and highly sensitive determination method for urinary free cortisol has been developed using reversed phase high performance liquid chromatography (HPLC) with a precolumn for sulphuric acidβethanol fluorescence derivatization and column switching. Urinary cortisol, eluted from t