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Determination of Triton X-100 binding to membrane proteins by polyacrylamide gel electrophoresis

โœ Scribed by Erik Fries


Book ID
115723013
Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
460 KB
Volume
455
Category
Article
ISSN
0005-2736

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Molecular weight estimation of Triton X-
โœ Vincent J. Hearing; Walter G. Klingler; Thomas M. Ekel; Paul M. Montague ๐Ÿ“‚ Article ๐Ÿ“… 1976 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 632 KB

A polyacrylamide gel elec&ophoresis system is described which employs the nonionic detergent T&on X-100 as a protein solvent. Aside from the obvious advantages of this detergent over ionic detergents and urea in the preservation of protein structure and function, it is demonstrated that the ancillar