✦ LIBER ✦

Determination of transferrin receptors on frozen sections of malignant B-cell lymphomas by immunofluorescence with a monoclonal antibody

✍ Scribed by Pieter B. Oudemans; Guy Brutel De La Rivière; Guus A. M. Hart; Peter van Heerde; Ger Scholte; Thea M. Vroom

Publisher: John Wiley and Sons
Year: 1986
Tongue: English
Weight: 707 KB
Volume: 58
Category: Article
ISSN: 0008-543X
DOI: 10.1002/1097-0142(19860915)58:6<1252::aid-cncr2820580613>3.0.co;2-m

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✦ Synopsis

A series of 34 B-cell lymphomas was studied for the presence of transferrin receptors by immunofluorescence on frozen tissue sections with a monoclonal antibody. These lymphomas were classified by light microscopic study and furthermore investigated by immunohistochemical and morphometric methods. There were diffuse centrocytic, diffuse centroblastic, and follicular and diffuse centrocytic/centroblastic lymphomas. Significant differences in log mean nuclear area in these four histologic groups were found, as well as a significant correlation between log nuclear area and transferrin receptors as measured semiquantitatively by fluorescence intensity of the cell membranes. In addition, it was found that there is a strong correlation between the transferrin receptor activity and malignancy grading on histologic and morphometric basis. Therefore, the transferrin receptor determination on frozen sections appears to be a good method of malignancy grading.

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