Mutated cytochrome b as a determinant of a new monoclonal antibody (H8.98) on renal carcinoma cell lines recognized by a Vγ3Vδ1+ T-cell clone
✍ Scribed by Anita Choudhary; Robert A. Kurt; Françoise Goret; Anne Moreau; Elisabeth Diéz; Walter J. Urba; Francine Jotereau; Christine Pourcel
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- French
- Weight
- 250 KB
- Volume
- 82
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
We generated a monoclonal antibody (MAb), H8.98, that recognizes an antigen shared by 50% of examined renal carcinoma cell (RCC) lines and is susceptible to lysis by a V␥3V␦1 ؉ T-cell clone derived from RCC tumor-infiltrating lymphocytes. H8.98 inhibited V␥3V␦1 ؉ T-cell clone-mediated lysis of RCC lines. It did not stain normal kidney lines, melanomas, fibroblasts, Burkitt's lymphoma or Epstein-Barr virus-transformed B-cell lines but it did stain 2 of 4 tested breast cancer lines. Through screening of a renal carcinoma cDNA library using H8.98, we isolated a cDNA clone which, upon sequencing, was found to be cytochrome b with 2 point mutations. Int.