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Determination of trace α-fetoprotein variant by affinity adsorption solid substrate-room temperature phosphorimetry

✍ Scribed by Jia-Ming Liu; Zhen-Bo Liu; Xue-Lin Li; Zhi-Ming Li; Xiao-Mei Huang; Feng-Shan Hong; Wei-Nv Lin; Fang Chen

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
524 KB
Volume
24
Category
Article
ISSN
1522-7235

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✦ Synopsis

Abstract

The 3.5‐generation dendrimers (3.5G‐D)–porphyrin (P) dual luminescent molecule (3.5G‐D–P) was used to label concanavalin agglutinin (Con A); the product of the reaction is 3.5G‐D–P–Con A. A new method for the determination of trace AFP‐V by affinity adsorption solid substrate–room temperature phosphorimetry (AA‐SS–RTP) was established, based on the room temperature phosphorescence (RTP) property of the product on polyamide membrane (PAM) substrate and the specific affinity adsorption (AA) reaction between 3.5G‐D–P–Con A and α‐fetoprotein variant (AFP‐V), which caused the RTP of the system to be sharply enhanced, the Δ__Ip__ was linearly correlated to the content of AFP‐V. The sensitivity of the method was obviously high. It could accurately detect the content of AFP‐V in serum. The results were tallied well with those obtained by the ELISA method. Copyright © 2008 John Wiley & Sons, Ltd.

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## Abstract A new solid substrate room temperature phosphorimetry (SSRTP) for the determination of trace mercury has been established, using Triton X‐100 as a sensitizer. The regression equation of working curve was Δ__I__~p~=11.40__m__(Hg^2+^)+1.569 (ag·spot^−1^, __n__=6, Δ__I__~p~=__I__~p1~−__I__