Solid Substrate Room Temperature Phosphorimetry for the Determination of Trace Mercury Based on the Reaction between Alkaline Phosphatase and Mercury Using Triton X-100 as a Sensitizer
✍ Scribed by Jiaming Liu; Feiming Li; Shaoqin Lin; Zhiming Li; Yayu Chen; Huiqing Lin; Lichai Huang; Jingping Cai; Xuejuan Chen
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 82 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0256-7660
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✦ Synopsis
Abstract
A new solid substrate room temperature phosphorimetry (SSRTP) for the determination of trace mercury has been established, using Triton X‐100 as a sensitizer. The regression equation of working curve was Δ__I__~p~=11.40__m__(Hg^2+^)+1.569 (ag·spot^−1^, n=6, Δ__I__~p~=I~p1~−I~p2~, I~p1~ and I~p2~ referred to the phosphorescence intensity of the blank reagent and the test solution, respectively), and correlation coefficient (r) was 0.9984. The RSD valus of the determination of 0.016 and 8.0 ag·spot^−1^ Hg^2+^ were 4.1% and 1.7% (n=8), respectively, indicating that the method had good repeatability. The limit of detection (LOD) calculated by 3Sb/k was 7.0 zg·spot^−1^ Hg^2+^ (corresponding concentration: 1.8×10^−17^ g·mL^−1^, Sb=0.025, n=11). This method has high sensitivity, selectivity and precision, which was applied to determination of trace mercury in water samples with the result being agreed very well with that of dithizone extraction spectrophotometry.