Determination of total free amino acids with o-phthalaldehyde and N-acetyl-l-cysteine
✍ Scribed by M.J.Medina Hernández; R.M.Villanueva Camañas; M.C.García Alvarez-Coque
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 305 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0026-265X
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This report examines the RP-HPLC separation of o-phthalaldehyde derivatives of amino acids, amino sugars, and amino sugar alcohols using either 2-mercaptoethanol or 3-mercaptopropionic acid. A method with pmol sensitivity for the analysis of N-acetylamino sugars of glycoconjugates was elaborated. Up
levels are easily obtainable, even in indoor environments with low smoking rates (e.g., restaurants and offices) where 1-h sampling typically yields values of total RSP in the 10-100 pg range [8].