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Determination of Thiopental by High Pressure Liquid Chromatography

โœ Scribed by Christensen, J. Heslop ;Andreasen, F.


Book ID
114799612
Publisher
Wiley (Blackwell Publishing)
Year
2009
Weight
240 KB
Volume
44
Category
Article
ISSN
0001-6683

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A rapid, precise, and simple HPLC method provides an assay of hydroxyproline from tissue extracts or solutions of collagen. Samples are hydrolyzed with 6 N HCl, derivatized with phenyl isothiocyanate, and chromatographed on a small, C18 reverse-phase HPLC column. Hydroxyproline (Hyp) is separated fr