Determination of the three-dimensional solution structure of Raphanus sativus Antifungal Protein 1 by 1H NMR
โ Scribed by Franky Fant; Wim Vranken; Willem Broekaert; Frans Borremans
- Book ID
- 115628546
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 537 KB
- Volume
- 279
- Category
- Article
- ISSN
- 0022-2836
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Aesculus hippocastanum antimicrobial protein 1 (Ah-AMP1) is a plant defensin isolated from horse chestnuts. The plant defensins have been divided in several subfamilies according to their amino acid sequence homology. Ah-AMP1, belonging to subfamily A2, inhibits growth of a broad range of fungi. So
Sequence-specific ~H and 15N resonance assignments have been made for all 145 non-prolyl residues and for the flavin cofactor in oxidized DesulJovibrio vulgaris flavodoxin. Assignments were obtained by recording and analyzing 1H-~SN heteronuclear three-dimensional NMR experiments on uniformly 15N-en