๐”– Bobbio Scriptorium
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Determination of the solubilizing activity of a cellulase complex with dyed substrates

โœ Scribed by Matti Leisola; Matti Linko

Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
371 KB
Volume
70
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

When the solubilizing activity of a microbial cellulase complex (e.g., Trichoderma viride) is determined with conventional methods based on formation of reducing sugars, the results depend on the concentration ratio of cellobiose and glucose in the reaction mixture and thus on the/3-glucosidase present and on the type of measurement of reducing sugars. The use of dyed substrates is one way to avoid this problem. The release of coloured compounds from a dyed substrate is proportional to the solubilization.

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Continuous photometric determination of
Continuous photometric determination of endo-1,4-ฮฒ-d-glucanase (cellulase) activity using 4-methylumbelliferyl-ฮฒ-d-cellobioside as a substrate
โœ Vladimir M. Chernoglazov; Aida N. Jafarova; Anatole A. Klyosov ๐Ÿ“‚ Article ๐Ÿ“… 1989 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 430 KB

A very simple and sensitive procedure for the determination of the activity of highly purified endo-1,4-beta-glucanase from the microscopic fungus Trichoderma reesei using 4-methylumbelliferyl-beta-D-cellobioside has been developed. The HPLC study has shown that this substrate is cleaved by endo-1,4