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Determination of the secondary structure in solution of the Escherichia coli DnaA DNA-binding domain

✍ Scribed by Takayuki Obita; Takafumi Iwura; Masayuki Su’etsugu; Yoichiro Yoshida; Yoshitsugu Tanaka; Tsutomu Katayama; Tadashi Ueda; Taiji Imoto


Book ID
117052867
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
233 KB
Volume
299
Category
Article
ISSN
0006-291X

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Flow cytometry was used to study initiation of DNA replication in Escherichia coli K12 after induced expression of a plasmid-borne dnaA+ gene. When the dnaA gene was induced from either the plac or the lambda pL promoter initiation was stimulated, as evidenced by an increase in the number of origins