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Determination of the regiochemistry of insect epoxide hydrolase catalyzed epoxide hydration of juvenile hormone by 18O-labeling studies

โœ Scribed by Russell J. Linderman; Elizabeth A. Walker; Carol Haney; R. Michael Roe

Publisher
Elsevier Science
Year
1995
Tongue
French
Weight
726 KB
Volume
51
Category
Article
ISSN
0040-4020

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A partition assay for the simultaneous d
A partition assay for the simultaneous determination of insect juvenile hormone esterase and epoxide hydrolase activity
โœ Marla R. Share; R.Michael Roe ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 694 KB

A partition assay was developed to measure insect juvenile hormone (JH) I and III metabolism in biological samples containing both JH esterase and JH epoxide hydrolase activity. The assay utilizes commercially available radiochain 3H-labeled JH as substrate and the selective JH esterase inhibitor 3-