A partition assay for the simultaneous determination of insect juvenile hormone esterase and epoxide hydrolase activity

✍ Scribed by Marla R. Share; R.Michael Roe

• Publisher: Elsevier Science
• Year: 1988
• Tongue: English
• Weight: 694 KB
• Volume: 169
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(88)90257-6

✦ Synopsis

A partition assay was developed to measure insect juvenile hormone (JH) I and III metabolism in biological samples containing both JH esterase and JH epoxide hydrolase activity. The assay utilizes commercially available radiochain 3H-labeled JH as substrate and the selective JH esterase inhibitor 3-octylthio-1,1,1-trifluoro-2-propanone. JH partitions into an isooctane phase and the metabolites JH acid, JH diol, and JH diol-acid into aqueous methanol after incubation of JH substrate with inhibited and uninhibited sample. The assay provides a time- and cost-efficient alternative to the currently available thin-layer chromatography method for the measurement of JH esterase and epoxide hydrolase activity.