Determination of the Extent of Protein Biotinylation by Fluorescence Binding Assay
β Scribed by Rao, Srivatsa V.; Anderson, Kimberly W.; Bachas, Leonidas G.
- Book ID
- 126401656
- Publisher
- American Chemical Society
- Year
- 1997
- Tongue
- English
- Weight
- 133 KB
- Volume
- 8
- Category
- Article
- ISSN
- 1043-1802
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π SIMILAR VOLUMES
## Abstract A practical class experiment is described to show the students an application of fluorescence spectroscopy in the study of proteinβligand binding. This class is part of an undergraduate physical biochemistry course for life science students. The major aim is to introduce the students to
Ribonuclease A (RNase A) and the ribonuclease inhibitor protein (RI) form one of the tightest known protein-protein complexes. RNase A variants and homologues, such as G88R RNase A, that retain ribonucleolytic activity in the presence of RI are toxic to cancer cells. Herein, a new and facile assay i