Determination of the degree of deacetylation of chitosan by capillary zone electrophoresis
β Scribed by Wu, Chunhung; Kao, Chen Yao; Tseng, Shih-Ying; Chen, Kuan Cheng; Chen, Shih-Feng
- Book ID
- 122111374
- Publisher
- Elsevier Science
- Year
- 2014
- Tongue
- English
- Weight
- 929 KB
- Volume
- 111
- Category
- Article
- ISSN
- 0144-8617
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This paper describes a novel method to determine the degree of deacetylation of chitosan by IH NMR spectroscopy. Measurements were carried out at 70~ by using 2 wt% CD3COOD/D20 and 2 wt% DCI/D20 as solvents for chitosan. In the case of DCI/D20 system, effect on hydrolysis of chitosan should be take
A new method was developed for the accurate determination of the degree of deacetylation of chitosan. The method involves the complete hydrolysis of chitosan to glucosamine and N-acetylglucosamine by a cooperative action of chitosanolytic enzymes exo-beta-D-glucosaminidase, beta-N-acetylhexosaminida