Determination of the cyanobacterial neurotoxin, anatoxin-a, by derivatisation using 7-fluoro-4-nitro-2,1,3-benzoxadiazole (NBD-F) and HPLC analysis with fluorimetric detection

✍ Scribed by Kevin J. James; Ian R. Sherlock

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 178 KB
• Volume: 10
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/(sici)1099-0801(199601)10:1<46::aid-bmc551>3.0.co;2-7

✦ Synopsis

A sensitive IIPLC analysis has been developed for the determination of anatoxin-a in cyanobacterial bloom material and in raw and treated waters. Efficient extraction of this toxin from its matrix can be achieved using solid-phase extraction with a weak cation exchanger. HPLC analysis with fluorimetric detection (& 470 nm, ACm 530 nm) was possible after derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F). This new analytical protocol permits the detection of anatoxin-a at low concentrations (0.1 pg/L) and gave a good linear calibration (1-20 pglL, r = 0.997).