Determination of the anomeric configuration of D-xylose with D-xylose isomerases
β Scribed by Hilda Kersters-Hilderson; Marc Claeyssens; Elisa Van Doorslaer; Clement K. De Bruyne
- Book ID
- 118519074
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 342 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0008-6215
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
An enzymic assay for the quantitative determination of D-XylOSe has been developed. The method is based on a coupled reaction of o-xylose isomerase with o-glucitol dehydrogenase. Perfect agreement between the results, obtained with the o-toluidine-acetic acid method and with the coupled enzymic assa
## Abstract A proposed reaction mechanism for the enzyme Dβxylose isomerase involves the ring opening of the cyclic substrate with a subsequent conformational rearrangement to an extended openβchain form. Restrained energy minimization was used to simulate the rearrangement. In the ringβopening ste