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Determination of the anomeric configuration of D-xylose with D-xylose isomerases

✍ Scribed by Hilda Kersters-Hilderson; Marc Claeyssens; Elisa Van Doorslaer; Clement K. De Bruyne


Book ID
118519074
Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
342 KB
Volume
47
Category
Article
ISSN
0008-6215

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Anomeric specificity of D-xylose isomera
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An enzymic assay for the quantitative determination of D-XylOSe has been developed. The method is based on a coupled reaction of o-xylose isomerase with o-glucitol dehydrogenase. Perfect agreement between the results, obtained with the o-toluidine-acetic acid method and with the coupled enzymic assa

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## Abstract A proposed reaction mechanism for the enzyme D‐xylose isomerase involves the ring opening of the cyclic substrate with a subsequent conformational rearrangement to an extended open‐chain form. Restrained energy minimization was used to simulate the rearrangement. In the ring‐opening ste