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Quantitative determination of d-xylose by a coupled reaction of d-xylose isomerase with d-glucitol dehydrogenase

✍ Scribed by Hilda Kersters-Hilderson; Elisa Van Doorslaer; Clement K. De Bruyne; Kei Yamanaka

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
499 KB
Volume
80
Category
Article
ISSN
0003-2697

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✦ Synopsis

An enzymic assay for the quantitative determination of D-XylOSe has been developed. The method is based on a coupled reaction of o-xylose isomerase with o-glucitol dehydrogenase. Perfect agreement between the results, obtained with the o-toluidine-acetic acid method and with the coupled enzymic assay, was found. The method has also been successfully used in a kinetic study of P-Dxylosidase using xylobiose as substrate. Accurate and reproducible results were obtained throughout all experiments.

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