𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Determination of tetrahydrocannabinolic acid—carrier protein conjugate by matrix-assisted laser desorption/ionization mass spectrometry and antibody formation

✍ Scribed by Yuri Goto; Yumi Shima; Satoshi Morimoto; Yukihiro Shoyama; Hiroki Murakami; Akihiko Kusai; Kazutetsu Nojima

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
348 KB
Volume
29
Category
Article
ISSN
1076-5174

No coin nor oath required. For personal study only.

✦ Synopsis

In order to form the monoclonal antibody against tetrahydrocannabinolic acid (THCA), THCA was coupled with bovine serum albumin (BSA) or human serum alhumin (HSA) using fl-alanine as a linker or without fl-alanine to produce individual antigen conjugates. The number of haptens contained in the antigen conjugates was determined by matrix-assisted laser desorption ionization mass spectrometry using BSA or HSA as an internal standard. The formation of monoclonal antibody is also discussed.

📜 SIMILAR VOLUMES

Determination of protein-protein interac
Determination of protein-protein interactions by matrix-assisted laser desorption/ionization mass spectrometry
✍ Farmer, Terry B.; Caprioli, Richard M. 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 279 KB 👁 2 views

A number of different procedures have been developed for use with matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS) for the analysis of non-covalent protein-protein complexes. These include use of specific matrix and laser combinations, accumulation of "first shot" spectra, mo

Analysis of wheat gluten proteins by mat
Analysis of wheat gluten proteins by matrix-assisted laser desorption/ionization mass spectrometry
✍ Dworschak, Ragnar G.; Ens, Werner; Standing, Kenneth G.; Preston, Ken R.; Marchy 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 237 KB 👁 2 views

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS) was used to analyze the protein composition in several common and durum wheat varieties. Mass spectra were obtained directly from crude and partially puriÐed wheat gliadin and reduced glutenin subunit fractions. Mass spectra of

Decomposition of protein nitrosothiolsin
Decomposition of protein nitrosothiolsin matrix-assisted laser desorption/ionization and electrospray ionization mass spectrometry
✍ Rina Kaneko; Yoshinao Wada 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 217 KB 👁 1 views

## Abstract The __S__‐nitrosylation of proteins is involved in the trafficking of nitric oxide (NO) in intra‐ and extracellular milieus. To establish a mass spectrometric method for identifying this post‐translational modification of proteins, a synthetic peptide and transthyretin were __S__‐nitros