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Determination of taurine in biological samples by reversed-phase liquid chromatography with precolumn derivatization with dinitrofluorobenzene

✍ Scribed by Zilin Chen; Gang Xu; Karl Specht; Rongjian Yang; Shiwang She

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
371 KB
Volume
296
Category
Article
ISSN
0003-2670

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✦ Synopsis

A rapid and sensitive method for the determination of taurine in human milk and urine by reversed-phase liquid chromatography (LC) has been developed. It involves precolumn derivatization with dinitrofluorobenzene in NaHCO, buffer (pH 9.01, catalysed by dimethyl sulphoxide, separation by LC on a PBondapak Phenyl column at 40Β°C with acetonitrile-water (1: 1) and 0.01 mol 1-l phosphate buffer (pH 5.5) as mobile phase and UV absorbance detection at 360 run. The peak area is proportional to the concentration of taurine in the range lo-80 pg ml-', the correlation coefficient being 0.9997. The detection limit in standard solutions is 0.1 pg ml-' (signal-to-noise ratio = 3). The relative standard deviations (n = 6) of retention time and peak area are 0.45% and 2.29%, respectively. The average recovery for human milk and urine are 95.8% and lOl.O%, respectively.

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