Quantitative determination of allantoin in biological fluids by reversed-phase high-pressure liquid chromatography

✍ Scribed by W. Tiemeyer; D. Giesecke

• Publisher: Elsevier Science
• Year: 1982
• Tongue: English
• Weight: 178 KB
• Volume: 123
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(82)90616-9

✦ Synopsis

Allantoin is quantitatively determined in biological fluids by reversed-phase high-pressure liquid chromatography. The proteins of blood plasma are precipitated by perchloric acid. Urine can be analyzed directly. The reproducibility was over 99%. The average recovery of added allantoin in blood was 96% and in urine 98%. The retention factor k' was 0.235.