A simple one-step method for the analysis of monosaccharides including galactosaminitol after acidic hydrolysis is described. The hydrolyzate was re- \(\mathrm{N}\)-acetylated and analyzed by high-performance anion-exchange chromatography and the sugars were detected by a pulsed amperometric detecto
Determination of sugars and alditols in tobacco with high performance anion-exchange chromatography
โ Scribed by Kuntian Tang; Lina Liang; Yaqi Cai; Shifen Mou
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 662 KB
- Volume
- 30
- Category
- Article
- ISSN
- 1615-9306
No coin nor oath required. For personal study only.
โฆ Synopsis
Abstract
Sugars, alditols, and alcohols in tobacco products were quantified utilizing a method of highโperformance anionโexchange chromatographyโpulsed amperometric detection (HPAECโPAD). The optimized analytical method can be used to classify different tobaccos and control the quality of tobaccos. To substantiate the applicability of the method, the analysis of 27 tobacco samples with satisfactory linearity, repeatability, and accuracy had been demonstrated. Compared with some other analytical methods for tobacco, the HPAECโPAD method provided a simple and powerful tool for the analysis of not only sugars but also alcohols in tobacco extracts.
๐ SIMILAR VOLUMES
High-performance anion-exchange (HPAE) chromatography under alkaline conditions (pH approximately 13) has been used to separate neutral oligosaccharides from human milk as well as oligosaccharide alditols isolated by alkaline borohydride degradation of O-linked glycoproteins having blood group A and
Cell cultures and fermentation broths are complex mixtures of organic and inorganic compounds. Many of these compounds are synthesized or metabolized by microorganisms, and their concentrations can impact the yields of desired products. Carbohydrates serve as carbon sources for many microorganisms,